Effects of maternal exposure to sevoflurane for anesthesia or surgery on social interaction behaviors in offspring mice / 中华麻醉学杂志

ABSTRACT

Objective To evaluate the effects of maternal exposure to sevoflurane for anesthesia or surgery on social interaction behaviors in offspring mice. Methods Fifteen pregnant C57BL∕6 mice on ges-tational day 18 were assigned into 3 groups(n=5 each)using a random number table control group (group C), sevoflurane anesthesia group(group Sevo)and surgery group(group Surg). Group C inhaled 100% oxygen for 2 h in group C. Group Sevo inhaled 3% sevoflurane in oxygen for 2 h. In group Surg, 3%sevoflurane in oxygen was inhaled for 2 h and exploratory laparotomy was performed. At juvenile age(on 30th day after births)and in early adulthood(on 60th day after births), the offspring mice underwent so-cial interaction tests using the three-chambered sociability paradigm, including sociability and preference for social novelty. Results During 1st testing phase there was no significant difference in time spent in ex-ploring the chamber on the left and right sides or in sniffing directed at the empty wire cage on the left and right sides among the three groups(P>005). During 2nd testing phase time spent in exploring the cham-ber containing stranger-1 mouse in the wire cage and in sniffing directed at the wire cage containing stranger-1 mouse was significantly prolonged at juvenile age and in early adulthood in three groups when compared with the empty wire cage(P<005). During 3rd testing phase compared with stranger-1 mouse, time spent in exploring the chamber containing stranger-2 mouse at juvenile age and in sniffing directed at the wire cage containing stranger-2 mouse at juvenile age and in early adulthood was significantly prolonged in group C, time spent in exploring the chamber containing stranger-2 mouse and in sniffing directed at the wire cage containing stranger-2 mouse was significantly prolonged in early adulthood in group Sevo, and time spent in sniffing directed at the wire cage containing stranger-2 mouse was significantly prolonged in early adulthood in group Surg(P<005). Conclusion Maternal exposure to sevoflurane for anesthesia or surgery induces social interaction deficits in the offspring mice in which lower levels of preference for social novelty are detected, especially at juvenile age.