Prevalence of Mycoplasma pneumoniae infection in children in Hengyang and comparison of three methods for genotyping / 中华微生物学和免疫学杂志
Chinese Journal of Microbiology and Immunology
;
(12): 862-868, 2017.
Article
in Chinese
| WPRIM
| ID: wpr-666283
ABSTRACT
Objective To investigate the prevalence of Mycoplasma pneumoniae ( Mp) infection in children in Hengyang from 2013 to 2016 and to analyze the p1 genotypes of the isolated Mp strains by using polymerase chain reaction-restriction fragment length polymorphism ( PCR-RFLP) , nested polymerase chain reaction (nPCR) and rapid-cycle polymerase chain reaction (Rapid-Cycle PCR).Methods Throat swab samples of children with acute respiratory tract infection were collected from four hospitals in Hengyang , Hu-nan Province from 2013 to 2016.Mp strains in these samples were identified by PCR amplification of the 16S rRNA gene.PCR-RFLP, nPCR and Rapid-Cycle PCR were performed for Mp p1 genotyping in order to fur-ther analyze the genotypes of Mp strains circulating in Hengyang .Results A total of 109 clinical strains of Mp were identified from the 984 throat swab samples .The sensitivities of PCR-RFLP and nPCR for genoty-ping MP strains were both 100%, while that of rapid-Cycle PCR was 98 .17%.All of the three methods showed 100%specificity for genotyping.Of all isolated Mp strains, 78.90% were p1 gene type Ⅰ and 21.10%were p1 gene typeⅡ(t=93.239, P=0.01).From 2013 to 2016, the annual isolation rates of p1 gene type Ⅰ and type Ⅱ strains were 93.10%, 87.5%, 76.92%, 65.79% and 6.90%, 12.5%, 23.08%, 34.21%, respectively.The rate of Mp p1 gene type Ⅰinfection decreased over year , while that of p1 gene type Ⅱinfection increased gradually .Conclusion PCR-RFLP, nPCR and rapid-Cycle PCR are reliable for genotyping of Mp p1 gene.The predominant genotype of Mp strains circulating in Hengyang is p 1 gene type Ⅰ, but the incidence of p 1 gene type Ⅱinfection gradually increases from 2013 to 2016 .
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Prevalence study
/
Risk factors
Language:
Chinese
Journal:
Chinese Journal of Microbiology and Immunology
Year:
2017
Type:
Article
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