A new methodology of DNA polymorphism detection from standard long gun / 中国法医学杂志
Chinese Journal of Forensic Medicine
; (6): 385-387,392, 2017.
Article
in Zh
| WPRIM
| ID: wpr-666629
Responsible library:
WPRO
ABSTRACT
Objective A new methodology was established to efficiently obtain the genotype of DNA remained on standard long gun. Methods Direct PCR and silicon membrane method were combined to detect DNA polymorphism of a total of 240 samples at 5 different positions from 48 standard long guns. Results Combining direct PCR and silicon membrane method, we obtained full DNA profiles in 42 out of 48 standard long guns, with a detection rate up to 87.50%. Conclusion The results demonstrate that the combination of direct PCR and silicon membrane method provide a quick and accurate way to detect DNA polymorphism on the standard long gun.
Full text:
1
Index:
WPRIM
Type of study:
Diagnostic_studies
Language:
Zh
Journal:
Chinese Journal of Forensic Medicine
Year:
2017
Type:
Article