A successful pregnancy using completely immotile but viable frozen-thawed spermatozoa selected by laser / 대한생식의학회지
Clinical and Experimental Reproductive Medicine
;
: 52-55, 2017.
Article
in English
| WPRIM
| ID: wpr-66665
ABSTRACT
The aim of this study was to report a successful pregnancy using completely immotile frozen-thawed spermatozoa selected by laser. A single laser shot was used to detect the presence of viable immotile spermatozoa in fresh and frozen-thawed testicular spermatozoa. The viability rate was 55.8% after the laser detection, and cryopreservation was carried out immediately. The thawing test was performed on the day of oocyte pick-up, and no motile sperm were observed after extending the culture for another 4 hours, while a survival rate of 39.8% was detected using the laser. In all, five mature oocytes were injected, resulting in four cases of normal fertilization (80%) on day 1. Further, two high-quality day 3 embryos were transferred, which resulted in a singleton pregnancy. Our study demonstrates that completely immotile spermatozoa are worth cryopreserving for further intracytoplasmic sperm injection, which provides a new insight into male fertility preservation in cases of completely immotile spermatozoa.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oocytes
/
Spermatozoa
/
Cryopreservation
/
Survival Rate
/
Sperm Injections, Intracytoplasmic
/
Embryonic Structures
/
Fertilization
/
Fertility Preservation
Limits:
Humans
/
Male
/
Pregnancy
Language:
English
Journal:
Clinical and Experimental Reproductive Medicine
Year:
2017
Type:
Article
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