Knockdown of survivin expression increases chemosensitivity of glioma cells to temozolomide / 解放军医学杂志

ABSTRACT

Objective To study the effect of survivin on temozolomide (TMZ) resistance of glioma cells and possible mechanism.Methods Survivin RNA and survivin shRNA plasmids were transfected into U87 cells,respectively.The cell growth curve drawn by MTT was used to test the changes in the proliferation of glioma cells.Colony forming assay was used to calculate cell surviving fraction.The Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) staining were applied to detect the apoptosis rate by flow cytometer (FCM).Western blotting was used to detect the expression of activated caspase-3.Results The cell proliferation and cell surviving fraction were inhibited by TMZ,and it was more significantly inhibited in TMZ plus down-regulation of survivin.Compared with parental cells,U87/sur si cells,treated by TMZ,showed a decrease in proliferation and colony forming efficiency and an increase in apoptosis rate,whereas U87/sur cells,treated by TMZ,showed an increase in proliferation and colony forming efficiency and a decrease in apoptosis rate.Western blotting showed that compared with TMZ alone,the expression of activated caspase-3 protein was promoted in TMZ plus down-regulation of survivin.Conclusion Down-regulation of survivin could enhance the sensitivity of glioma cells to TMZ,which may be related to survivin promoting cell apoptosis.