Effects of Treadmill Running on Intramyocellular Lipid Deposition and VEGFB Expression in Soleus Muscle of High-fat Diet Mice / 中国运动医学杂志

ABSTRACT

Objective To observe the effect of treadmill running on skeletal muscle lipid deposition,and the expression of vascular endothelial growth factor-B(VEGFB)and its receptor in soleus muscles of high-fat diet mice.Methods Thirty-two 5-week-old C57BL/6 male mice were randomly divided into a control group(n=8)and a high-fat diet group(n=24),and fed with normal and high-fat diet respectively.Eight weeks later,16 obesity mice were selected from the latter group and randomly divided into a sedentary group(n=8)and a treadmill running group(n=8).The running group underwent treadmill running at 25 m/min for an hour every day,five days a week for 8 weeks,while the other two groups did not do any exercises.The weight was measured before the mice were killed.The soleus lipid deposition level was determined using oil red O staining,The expression of VEGFR1 and Neuropilin-1(NRP1)was determined using the immunohistochemical staining,while the mRNA expression of VEGFB,VEG-FR1,NRP1 and peroxisome proliferator-activated receptor-γcoactivator-1α (PGC-1α) was detected using the real-time PCR.Results Compared with the control group,a significant increase was observed in the weight and the soleus lipid deposition of the high-fat diet group,while there were no significant differences in the expression of VEGFB,NRP1,VEGFR1 and PGC-1α.mRNA between the two groups.Compared with the sedentary obese mice,significant decrease in the body weight and soleus lipid deposition,significant increase in VEGFR1 mRNA expression,but no significant changes in the expression of VEGFB,NRP1 and PGC-1α mRNA were observed in the treadmill-running obese mice.Conclusion The treadmill running has no effect on the VEGFR1 expression in soleus of obese mice,but significantly up-regulates the expression of its receptors.It indicates that the treadmill running can lower the intramyocellular lipid deposition as it can strengthen the VEGFB/VEGFR1 in soleus muscles.