Effects of miR-21 Antisense Oligonucleotides and Cisplatin Combined on the Growth of Osteosarcoma Xenotransplants in Nude Mice / 华中科技大学学报(医学版)

ABSTRACT

Objective To explore the effects of miR-21 antisense oligonucleotides(ASO)and cisplatin combined on the growth of subcutaneous xenotransplanted osteosarcomas in nude mice.Methods Xenotransplanted osteosarcoma models were established in nude mice by injection of human osteosarcoma cells MG-63.Inoculated mice were randomly divided into four groups(blank control group,cisplatin group,anti-miR-21 group,anti-miR-21+cisplatin group).The rats were treated by injec-tion of anti-miR-21 into the tumors in anti-miR-21 group,by injection of cisplatin through tail veins in cisplatin group or by in-jection both of them in anti-miR-21+ cisplatin group.The volume and weight of tumors were measured and the inhibition rates of tumors were calculated on a daily basis.Meanwhile,MG-63 cells were divided into the same four groups as mentioned be-fore.The cell proliferation was detected by MTT assay and the cell apoptosis by Annexin V analysis.Results The inhibition of tumor growth(volume and weight)was obvious in the anti-miR-21+cisplatin group compared to the control groups.In vitro cell growth was significantly inhibited and cell apoptosis increased obviously in the anti-miR-21 + cisplatin group compared to the control groups(P <0.05).Conclusion MiR-21 ASO combined with cisplatin can inhibit cell proliferation,promote cell apopto-sis in vitro ,and obviously suppress tumor growth in vivo.They may be used to treat osteosarcoma.