Effects of Xiaoyu Sanjie Yi'ai Enema on Gene PTEN Expression in Nude Mouse Model of Prostate Cancer / 广州中医药大学学报

ABSTRACT

Objective To investigate the effects of Xiaoyu Sanjie Yi'ai Enema on the expression level of phosphatase and tensin homolog deleted on chromosone 10(PTEN)gene of nude mouse model of prostate cancer, and to explore its anticancer mechanism. Methods Nude mouse model of prostate cancer was established by neck subcutaneous injection of suspension of human prostate cancer cells PC-3. Thirty tumor-bearing nude mice were randomly divided into control group and treatment group , 15 mice in each group. Ten days after modeling, the treatment group was treated with enema of Xiaoyu Sanjie Yi'ai Enema, and the control group was treated with enema of the same volume of normal saline,once every other day,for 30 continuous days. At the end of medication, the tumors were extracted completely, and then the tumor mass of the two groups was detected before and after treatment to calculate the tumor-inhibition rate. The mRNA and protein expression levels of PTEN gene were detected by the method of reverse transcription real-time quantitative polymerase chain reaction(RT-qPCR)and western blotting method,respectively. Results Tumor mass in the treatment group was reduced as compared with that in the control group (P < 0.05). The tumor-inhibition rate of the treatment group was 18.71%. RT-qPCR results showed that the relative expression level of the PTEN mRNA in the treatment group was higher than that in the control group, the difference being statistically significant (P < 0.001). Western blotting result showed that the relative protein expression level of PTEN in treatment group was (0.55 ± 0.13)and that in the control group was(0.32 ± 0.09)separately,the difference being statistically significant(P<0.001). Conclusion Xiaoyu Sanjie Yi'ai Enema has therapeutic effects on prostate carcinoma,and the molecule mechanism may be related with the up-regulation of PTEN gene mRNA expression and protein translation,thus to inhibit PC-3 cell proliferation and metastasis.