Concentration Determination of Isavuconazole in Rabbit Cornea by HPLC / 中国药房

ABSTRACT

OBJECTIVE:To establish a method for the concentration determination of isavuconazole in rabbit cornea. METH-ODSHPLC was performed on column of Waters X-Bridge C18 with mobile phase of 0.2% glacial acetic acid-acetonitrile(4951, V/V)at flow rate of 0.7 mL/min,column temperature was 40 ℃,detection wavelength was 284 nm,and injection volume was 10μL. Fifteen Japanese white rabbits with big ears were selected,50 μL of 0.2% isavuconazole suspension was dropped into eyes, then every 3 rabbits were executed after 5,15,30,60,90 min of administration and cornea samples were taken respectively. The determination of isavuconazole were determined after treating. RESULTS:The isavuconazole in leaching solution showed good lin-ear range in 0.0312-2.24μg/mL(r2=0.9999);oethod recovery rate was 91.7%-105.6%(RSD≤6.88%,n=5);extraction recov-ery rate was 100.50%-106.10%(RSD≤2.16%,n=3);and RSDs of inter-day(n=3)and intra-day(n=3)precision and stabili-ty(n=5)tests were lower than 10%. CONCLUSIONS:The method shows strong specificity,high accuracy,good reproducibili-ty,and can rapidly and accurately determine the concentration of isavuconazole in rabbit cornea.