Dual-modality imaging-guided sonodynamic therapy with DVDMS-Mn-loaded liposomes / 中华核医学与分子影像杂志

ABSTRACT

Objective To fabricate DVDMS-Mn-LPs and evaluate its potential on fluorescence mo-lecular imaging, MRI and sonodynamic therapy ( SDT) . Methods DVDMS was used to chelate with Mn2+, and then encapsulated into nanoliposomes by a typical thin-film rehydration method to fabricate DVDMS-Mn-LPs. The particle morphology, average diameter, zeta potential, encapsulation efficiencies and Mn2+content were determined. Fluorescence molecular imaging was performed on different concentrations of DVDMS-Mn-LPs (0, 40, 80, 120, 160 μg/ml) with the small animal fluorescence imaging system. T1WI was per-formed with 3.0 T MR. The SDT effect of DVDMS-Mn-LPs was verified by CCK8 assay among the following groupscontrol group, ultrasound group ( experimental group 1, EG1) , DVDMS-Mn-LPs group ( EG2) and DVDMS-Mn-LPs + ultrasound group ( EG3) . One-way analysis of variance and the least significant differ-ence t test were used to analyze the data. Results DVDMS-Mn-LPs exhibited a well-defined spherical mor-phology and homogeneous distribution. The encapsulation efficiency was (65.56±1.47)%. ICP-AES meas-urement revealed that the chelation of Mn2+ with DVDMS occurred at a molar ratio of 1. 61. The fluores-cence intensities progressively increased with the elevated concentrations of DVDMS-Mn-LPs. The r1 value for DVDMS-Mn-LPs was 23.74 mmol·L-1·s-1. The cell viabilities of EG3 was (54.82±8.55)%, which were significantly lower than those of EG2 and EG1 ((86.54±2.67)% and (83.76±6.48)%;F=6011, t values -8.35, -9.15, all P<0.001). Conclusion DVDMS-Mn-LPs is successfully fabricated and has good potential on fluorescence molecular imaging, MRI and SDT, which provides a promising imaging-guided modality for glioma treatment in vivo.