Study on the Preventive Effect of Resveratrol on Model Mice and Improvement Effect on Model Golden Hamsters with Hyperlipidemia / 中国药房

ABSTRACT

OBJECTIVE:To study the preventive effect of resveratrol on model mice and improvement effect on model golden hamsters (shorter for hamsters) with hyperlipidemia. METHODS:Hyperlipidemia animal models were induced by using high-fat and high-cholesterol diet. Mice and hamsters were both randomly divided into model group and resveratrol low-dose, medi-um-dose,high-dose groups(40,80,160 mg/kg for mice,25,50,100 mg/kg for hamsters),10 in each group. 10 corresponding animals were selected as normal control group. Except that normal control group was fed normal diet and intragastrically administrat-ed normal saline,other mice were intragastrically administrated relevant medicines when fed high-fat and high-cholesterol diet;oth-er hamsters were firstly modeled for 2 weeks and intragastrically administrated relevant medicines after modeling. High-fat and high-cholesterol diet was fed during administration. It was administrated for 4 weeks. Total cholesterol(TC)and low density lipo-protein cholesterol(LDL-C)levels in serum of mice and hamsters were detected every week. After 4 weeks,pre-protein converting enzyme subtilisin 9(PCSK9)mRNA and protein expression,microRNA-27a(miRNA-27a)expression in serum of mice and ham-sters,and low density lipoprotein receptor(LDLR)protein expression in liver tissue of mice were detected. RESULTS:Compared with normal control group,the TC,LDL-C,PCSK9 mRNA and protein,miRNA-27a levels of mice in model group were obvious-ly increased after 2 weeks of administration (P<0.05);LDLR protein level in liver tissue was obviously decreased (P<0.05). TC,LDL-C,PCSK9 mRNA and protein,miRNA-27a levels of hamsters in model group were obviously increased (P<0.05). Compared with model group,above-mentioned indexes of mice and hamsters in each administration group were obviously im-proved(P<0.05),which were positively correlated with dose. CONCLUSIONS:Resveratrol has preventive effect on model mice and improvement effect on model golden hamsters with hyperlipidemia. The mechanism may be down-regulating miRNA-27a ex-pression and PCSK9 protein expression in serum,to increase LDLR protein level in liver tissue,and finally reduce LDL-C level.