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Development of a New TaqMan-MGB Probe-Based Real-Time qPCR Method for the Detection of HCV-RNA / 现代检验医学杂志
Journal of Modern Laboratory Medicine ; (4): 32-35, 2017.
Article in Chinese | WPRIM | ID: wpr-667162
ABSTRACT
Objective Developing a rapid and accurate real-time qPCR method for the detection of HCV-RNA.Methods HCV nucleotide sequence was analysed in Clustal software and primers and probe were designed in the conserved region of 5'UTR.The reaction system optimization of real-time qPCR method was used chessboard titration,pseudoviral particles were used as quantitative standard to assess the performance.New methods was compared with clinical commonly used kit of HCV-RNA and discuss the application value.Results The sensitivity of new real-time qPCR method was 50 IU/ml,coefficient variation was less than 5%.The quantitative results of this method could be traceable to national standards of GBW09151a.40 samples were determined by new methods and clinical commonly used kit of HCV-RNA,the positive concordance rate was 100 %,the negative concordance rate was 56 %.14 samples were positive by new method,but negative by Qiagen kit,illustrating that the sensitivity of new method was superior to Qiagen kit.Conclusion New TaqMan-MGB probe-based real-time qPCR method is a specific,sensitive,simple,rapid and exactly used to detection of HCV-RNA.

Full text: Available Index: WPRIM (Western Pacific) Type of study: Diagnostic study / Practice guideline Language: Chinese Journal: Journal of Modern Laboratory Medicine Year: 2017 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Type of study: Diagnostic study / Practice guideline Language: Chinese Journal: Journal of Modern Laboratory Medicine Year: 2017 Type: Article