Influence of up-regulated PTEN gene expression on apoptosis and chemosensitivity of ovarian cancer cells / 中国医师杂志

ABSTRACT

Objective To explore the influence of up-regulated PTEN gene expression on apoptosis and chemosensitivity of ovarian cancer cells.Methods HEY cells transfected by exogenous PTEN plasmid (PTEN-HEY) were used as the PTEN-HEY group,and those transfected by empty vectors (pcDNA-HEY) and non transfected cells (HEY) were used as the control groups.The expressions of PTEN protein and mRNA in each group were detected with Western blot and quantitative real-time polymerase chain reaction (qRT-PCR),the influence of PTEN overexpression on HEY cells apoptosis was evaluated with flow cytometry,and tumorigenicity in vivo was evaluated with nude mouse model tumor formation test,and cisplatin sensitivity of tumor cells was evaluated with methyl thiazolyl tetrazolium (MTT) detection in condition of PTEN overexpression.Results Compared to those in the pcDNA-HEY group and HEY group,PTEN protein and mRNA expression levels significantly increased after PTEN gene transfected (PTEN-HEY group) in 24 h.Compared to those in the pcDNA-HEY group and HEY group,the apoptosis rate of PTEN-HEY group increased significantly.Transfected cells were inoculated into nude mice,and there were transplanted tumors at inoculated sites of all mice,and tumor growth in the PTEN-HEY group was significantly lower than that in the pcDNA-HEY group,and the weight was significantly lower than that in the pcDNA-HEY group.At 4th,5th and 6th day of the cells cultured with 4 μg/ml cisplatin,cell survival rate in the PTEN-HEY group was significantly lower than those in the HEY and pcDNA-HEY groups.Conclusions Up-regulating PTEN gene expression can promote apoptosis in HEY of human ovarian cell line,and increase the sensitivity to cisplatin chemotherapy.