Expression and clinical significance of serum HMGB1 and inflammatory factors in patients with spinal cord injury / 重庆医学

ABSTRACT

Objective To investigate the expression and clinical significance of serum high-mobility group box 1 (HMGB1) in the patients with spinal cord injury (SCI).Methods A total of 87 cases of acute SCI treated in the Aksu Corps First Division Hospital from January 2007 to January 2014 were collected as the SCI group.The SCI severity was evaluated according to the damage grade by the American Spinal Cord Injury Association(ASIA).Venous blood was collected on 1,3,7 d after SCI.Other 87 healthy adults served as the control group.The expression levels of serum HMGB1,inter1eukin-6 (IL-6),IL-1β and tumor necrosis factor alpha (TNF-a) in the two groups were measured by ELISA.Then the correlation between the HMGB1 expression level with IL-1β,IL-6 and TNF-α and the relation between HMGB1 with SCI severity were analyzed.Results The HMGB1,IL-6,IL-1 and TNF-α levels on 1,3,7 d after SCI in the SCI group were higher.The expression levels of serum HMGB1 and IL-6 on 1 d after SCI in the SCI patients were significantly increased,and reached the peak value on 3 d;the levels of IL-1β and TNF-α on 1 d after SCI were highest,and gradually decreased on 3,5 d.The correlation analysis showed that the expression levels of HMGB1 on 1,3 d after SCI were positively correlated with the IL-6,IL-1β and TNF-α expression levels(P＜0.05).The serum HMGB1 expression level on 1,3 d after SCI was closely correlated with the SCI severity (P＜0.05).The severe the SCI severity,the higher the serum HMGB1 expression level.Conclusion The expression level of serum HMGB1 in the patients with early SCI is significantly increased,and can be used as one of indicators for assessing the severity of early SCI.