Aminoglycoside resistance genes of Acinetobacter baumannii / 中国人兽共患病学报

ABSTRACT

To investigate the distribution of the resistance genes of Acinetobacter baumannii to aminoglycoside,48 strains of extensively drug resistant Acinetobacter baumannii were collected from the First Affiliated Hospital of Bengbu Medical College from January to December,2015.The drug sensitivity test and identification were performed by VITEK 2 compact automatic microorganism instrument.Twelve aminoglycosides modifying enzymes,three 16SrRNA methylase genes and efflux pump abeB gene were detected from these isolates by PCR.Results showed that among these experimental 16 genes,aac(6')-Ⅰb gene was detected from 19 of 48 isolates (39.6％),both armA and adeB genes were 43 (89.6％),ant(3")-Ⅰa gene was from 5 (10.4％),while the other genes were not found.And more than two gene types were amplified from 39 of 48 strains (81.3％).In conclusion,the aac(6')-Ⅰb,armA gene and efflux pump adeB may play a key role in drug resistance to aminoglycosides antibiot ics of Acinetobacter Baumanni in our hospital.