Tanshinone Ⅱ A Induce Human Small Cell Lung Cancer Cells Apoptosis and Molecular Mechanism / 医学研究杂志

ABSTRACT

Objective To know the Tanshinone Ⅱ A induce human small cell lung cancer cells (H446 cells) apoptosis,and discuss the possible molecular mechanism.Methods Determined by MTT method of different concentrations of Tanshinone Ⅱ A impact on human small cell lung cancer H446 cells proliferation;Determined by Hoechst 33258 method of different concentrations Tanshinone Ⅱ A influence on human small cell lung cancer H446 cells apoptosis;Determined by qPCR method of different concentrations of Tanshinone Ⅱ A impact on human small cell lung cancer H446 cells apoptosis gene;Determined by Westen blot method of different concentrations of Tanshinone lⅡ A effect on lung cancer H446 cells apoptosis.Results Different drug concentrations of Tanshinone Ⅱ A (0.3,0.6,1.25,2.5,5 and 10μg/ml) inhibit human small cell lung cancer H446 cells proliferation and depend by the concentration and time;With the increase of the concentration of the drug in the formation of human small cell lung cancer H446 cells apoptosis corpuscle number increase,significant difference was found in high concentration group (P ＜ 0.05);High concentration group of Tanshinone Ⅱ A promote apoptosis gene (Bax,caspase-9,caspase-3) expression,and inhibit apoptosis inhibiting genes (Akt,the Bcl-2) expression and there were significant differences (P ＜ 0.05);High concentration group of Tanshinone Ⅱ A promoting apoptosis protein (Bax,caspase-9,caspase-3)expression,and inhibit apoptosis inhibiting protein (Akt,the Bcl-2) expression and there were significant differences (P ＜ 0.05).Conclusion Tanshinone Ⅱ A may be through Akt-Bax/Bcl-2-caspase-9-caspase-3 signaling pathways induced human small cell lung cancer H446 cells apoptosis.