The protective effects of 4-hydroxybenzaldehyde on cerebral ischemia / reperfusion injury / 中国药理学通报

ABSTRACT

Aim To verify the mechanisms underlying the protective effects of the 4-hydroxybenzaldehyde (4-HBd)on cerebral ischemia / reperfusion in vivo and in vitro. Methods The model of focal cerebral ischemi-a / reperfusion in rats was established by the suture-oc-clusion method. After reperfusion,neurological score and cerebral infarction rate were assessed. Meanwhile, after pretreatment with 4-HBd for 24 h,the primary cultured cortical neuron cells were exposed to oxygen glucose deprivation / reoxygenation (OGD/ Rep). The survival rate, MDA, SOD, NO, Bax, Bcl-2 and caspase-3 concentrations were assessed. Results Pre-treatment with 4-HBd significantly decreased neurologi-cal score (P < 0. 05)and cerebral infarction rate (P <0. 01)in the cerebrum after MCAO/ R injury. Further-more,4-HBd significantly increased cell survival rate (P < 0. 05),decreased MDA content (P < 0. 05),in-creased SOD activity (P < 0. 05),decreased NO levels (P < 0. 05),decreased caspase-3 (P < 0. 05),in-creased Bcl-2 (P < 0. 05)but decreased Bax (P <0. 05)levels and therefore increased Bcl-2 / Bax ratio in the cerebrum after OGD/ R injury. Conclusions 4-HBd has significant protective effect on MCAO/ R rats, and the mechanism may be related to decreasing NO levels,Bax,Bcl-2,caspase-3 and reducing neuronal apoptosis.