Effects of human papillomavirus E5 gene on EGFR, p21, p53 and Rb gene expression in immortalized oral epithelial cells / 安徽医科大学学报

ABSTRACT

Objective To investigate the effects of HPV16 E5 on the expression of EGFR,p21,p53 and Rb in the human immortalized oral epithelial cell(HIOEC),and to discuss the potential mechanism of HPV16 E5 in the occurrence and development of oral squamous cell carcinoma.Methods Open reading frame of HPV16 E5 was cloned into the lentiviral vector pLOV-N to prepare recombinant plasmid pLOV-E5,which was stably transfected into HIOEC.EGFR,p21,p53 and Rb were detected by RT-qPCR and Western blot on the mRNA level and the protein level,respectively.Cell proliferation after stable trasfection was evaluated by MTT assay.Results After stable trasfection of HPV16 E5,the protein levels of EGFR,p21 and p53 in HIOEC were markedly overexpressed,as well as the mRNA levels of p21 and p53 (P =0.00,0.02).However,the mRNA levels of EGFR had no obvious change after trasfection.There were also no significant changes of Rb on the mRNA and protein levels.Compared with the control ceils,the proliferation activity of HIOEC/pLOV-E5 cells was significantly increased (P =0.00).Conclusion HPV16 E5 can stimulate the expression of EGFR,p21 and p53 as well as the proliferation of HIOEC.