Therapeutic effects of extract of Albizia chinensis(Osbeck)Merr. on rats with acetic acid-induced chronic gastric ulcer and anti-inflammatory mechanism / 国际药学研究杂志

ABSTRACT

Objective To investigate the anti-ulcer effects of Albizia chinensis(Osbeck)Merr. extract on rats with acetic acid-induced chronic gastric ulcer and explore the anti-inflammatory mechanism. Methods Wistar male rats(n=50)were randomized in-to five groupscontrol,model,famotidine,Albizia chinensis extract 100 and 200 mg/kg groups. In the experiment,30%acetic acid was used to induce chronic gastric ulcer in rats. The anti-ulcer effects of A. chinensis extract were evaluated by measuring the changes in general behavior of rats,gastric tissue morphology and the ulcer area after 12 d treatments. Wistar male rats(n=48)were random-ized into three groupscontrol,model and A. chinensis extract(200 mg/kg)groups. At 3 d after operation,the animals were sacrificed and stomachs were rapidly removed and processed for subsequent assays. The contents of TNF-αand IL-6 were evaluated by ELISA. The activity of myeloperoxidase(MPO)was tested by o-dianisidine method. Furthermore Western blot was used to examine the phos-phorylation of P38 and JNK. Results Compared with the model group,the gastric ulcer areas of rats were reduced by 38.4%(P<0.05)and the activity of MPO declined by 37.3%(P<0.01)in A. chinensis extract(200 mg/kg)group. Meanwhile the contents of TNF-αand IL-6 in A. chinensis extract(200 mg/kg)group respectively decreased by 43.8%(P<0.01)and 32.7%(P<0.01)com-pared with the model group. A. chinensis extract(200 mg/kg)obviously suppresed the phosphorylation of P38(P<0.01),but phos-phorylation of JNK and expression of P38 and JNK remained nearly unaltered. Conclusion A. chinensis extract(200 mg/kg)exhibits a protective effect on acetic acid-induced chronic gastric ulcer,which might be relevant to anti-inflammation by inhibiting the p38 sig-naling pathway but not JNK signaling pathway. A. chinensis extract(200 mg/kg)has no effect on JNK signaling pathway.