Influencing factors for extracting total polysaccharides and active adjuvant components from Poria cocos / 国际药学研究杂志

ABSTRACT

Objection To investigate the main influencing factors for extracting total polysaccharides and two active adjuvant components from Poria cocos. Methods Extracting temperature was designed between 20℃-100℃,ratio of material/water was 1/5-1/20 (W/V),extracting time was 1-6 h,extracting frequency was 1-3 times,material size was crude slices~50 mesh,and extracting mode was standing or stirring. The sugar content was determined by phenol-sulfuric acid method and the reletive molecular mass of polysac-charides was determined with gel filtration chromatography. Monosaccharide compositions were analyzed with PMP-derivation and cap-illary electrophoresis. Results ①The extracting temperature should be below 70℃;②The ratio of material/water was suitable be-tween 1/5-1/20;③The extracting time might be within 2 h;④The extracting frequency should be no more than two times;⑤The raw material should be crushed before extracting;⑥The stirring was favorable for extracting polysaccharides. Conclusion The re-sults indicate that the extracting temperature,extracting frequency and stirring mode are important for extracting polysaccharides,and the cushing of the raw materials is helpful to extract the active adjuvant component of polysaccharides from P. cocos in higher yield.