Isolation and Identification of Human Ovarian Cancer Stem-like Cells Induced by Short-term Treatment with Carboplatin / 中国医科大学学报
Journal of China Medical University
;
(12): 713-719, 2017.
Article
in Chinese
| WPRIM
| ID: wpr-668244
ABSTRACT
Objective To isolate carboplatin-resistant human ovarian cancer stem-like cells and provide a cellular model for the development and screening of second-line drugs for carboplatin-resistant ovarian cancer.Methods Primary ovarian cancer cells were isolated and cultured from ovarian cancer tissues and ascites,and ovarian cancer stem-like cell spheres were isolated from serum-free suspension culture directly or after carboplatin induction.The expression of stem cell markers was evaluated by immunofluorescent assay or flow cytometry.Results The ovarian cancer stem-like cell spheres were isolated successfully from both ovarian cancer tissues and ascites and expressed high levels of stem cell markers CD133,ALDH1,ABCG2,and CD24.The abundance of CD24+ cells in the ovarian cancer stem cells was significantly increased upon short-term induction with carboplatin.Condusion Ovarian cancer stem-like cells can be enriched and purified by short-term induction with carboplatin.This cell model can be used in research on chemoresistant ovarian cancer in the future.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
Language:
Chinese
Journal:
Journal of China Medical University
Year:
2017
Type:
Article
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