Surveillance of antibiotic resistance of clinical isolates in Shandong Provincial Hospital during 2016 / 中国感染与化疗杂志

ABSTRACT

Objective To investigate the antibiotic resistance of clinical isolates in Shandong Provincial Hospital during 2016.Method The antimicrobial susceptibility of isolates was tested by using VITEK 2-Compact system or disk diffusion method.All the data were analyzed by WHONET 5.6 software according to CLSI 2016 breakpoints.Results A total of 4 810 non-duplicate clinical strains were collected during 2016,of which gram-negative bacilli and gram-positive cocci accounted for 70.2％ and 29.8％,respectively.The most common specimen source was respiratory tract (38.1％),followed by skin and soft tissue (21.1％) and urine (17.5％).Escherichia coli was the most frequently isolated bacteria,followed by Staphylococcus aureus,Pseudomonas aeruginosa,Klebsiella and Enterococcus,accounting for 24.3％,10.2％,9.9％,9.8％ and 9.6％,respectively.The prevalence of ESBLs-positive E.coli and K.pneumoniae was 55.0％ and 37.9％,respectively.Carbapenem-resistant E.coli and K.pneumoniae accounted for 1.6％ and 4.2％,respectively.The percentage of P aeruginosa resistant to imipenem and meropenem was 24.3％ and 21.9％,respectively.More than 40％ of the A.baumannii strains were resistant to all the antibiotics tested except minocycline (33.2％).The prevalence of beta-lactamase positive strains was 70.1％ in H.influenzae and 97.5％ in M.catarrhalis.The prevalence of methicillin-resistant S.aureus (MRSA) was 31.4％.No staphylococcal strains were found resistant to vancomycin,teicoplanin or linezolid.E.faecium strains showed higher resistance rate to most antibiotics tested than E.faecalis.One strain of E.faecium was resistant to both vancomycin and teicoplanin.A total of 191 strains of S.pneumoniae were isolated,of which 143 (74.9％) isolates were from pediatric wards.None of the non-meningitis strains was resistant to penicillin.Other hemolytic Streptococcus strains were sensitive to penicillin,cephalosporins and vancomycin.Conclusions Bacterial resistance is still on rise.We should pay more attention to strengthening antimicrobial resistance surveillance and improving rational use of antimicrobial agents.