Effects of dasatinib on the proliferation and invasion and metastasis of breast cancer / 中国现代普通外科进展

ABSTRACT

Objective:Our study attempts to investigate the effects and the mechanism of dasatinib on the proliferation,invasion and metastasis of triple-negative breast cancer (TNBC) cells.Method:TNBC Hs578T cells were tested for the sensitivity to dasatinib in vitro using PrestoBlueTM reagent and mammosphere assay.Transwell assay and wound-healing assay were used to determine invasion and metastasis ability of Hs578T cells.The p-STAT3 and STAT3 expressions of dasatinib]treated Hs578T cells were measured by Western blot.Results:Dasatinib exhibited growth inhibition of Hs578T cells.Mammosphere assay showed that dasatinb treatment led to less mammosphere formation of TNBC cells.More importantly,migration and invasion ability of Hs578T cells was also decreased by dasatinib treatment.Western blot analysis revealed that the p-STAT3 level was decreased significantly in Hs578T cells after dasatinib treatment,compared with control group.Conclusions:Dasatinib prevents the proliferation,invasion and metastasis ability of TNBC cells via inhibiting STAT3 pathway.