The expression of LASS2 in bladder cancer and its relationship with tumor proliferation and apoptosis in nude mice / 医学研究生学报

ABSTRACT

Objective Bladder cancer is one of the most common malignant tumors involving urinary system , yet its pathogene-sis has not been fully and thoroughly studied .The study aimed to de-tect the expression of LASS 2 in bladder cancer model of nude mice and investigate the relationship of LASS 2 with tumor proliferation and apoptosis as well as its possible molecular mechanism . Methods Tumor development in nude mice was observed through the establish-ment of orthotopic bladder cancer model by transplantation , bladder cancer metastasis model by subcutaneous injection and blank con-trol group.LASS2 expression and changes in proliferation and apoptosis were detected in tumor tissues of different parts . Results Bladder cancer cell injected subcutaneously metastasis model tumor formation rate of 100%.The two models were not found transfer phenomenon in vivo.Compared with blank control group (81.0%), LASS2 expression (60.0%, 14.0%) was significantly decreased in the inoculated group and subcutaneous implantation group ( P<0.05) .Compared with the blank control group ( 16.0%) , the expression of Ki67 in the inoculated group and subcutaneous implantation group increased (50.0%and 78.0%) (P<0.05).Compared with the in situ perfusion group, the expression of LASS2 (14.0%) was significantly decreased (P<0.05) and the expression of Ki67 (78.0%) was increased (P<0.05).Compared with the blank control group , the expression of Bcl-2 in subcutaneous implantation group and in si-tu perfusion group was significantly increased ( P<0.05) .Compared with the subcutaneous implantation group , the expression of Bcl-2 was increased in the in situ perfusion group ( P<0.05) , while the expression of Bcl-x1 in the in situ implanted tumor was higher than that in the other two groups (P<0.05).The expression level of Bax and caspase3 in each group was not statistically significant (P>0.05) .Compared with the blank control group , the expression of Bim was significantly decreased in the subcutaneous implantation group (P<0.05). Conclusion The expression of LASS2 may be related to the tumorigenicity , proliferation and apoptosis in EJ blad-der cancer cells .