The protective effects of ulinastatin on brain tissue after CPR in swine / 中华急诊医学杂志

ABSTRACT

Objective To study the protective effects of ulinastatin on brain tissue from pathomorphism and the changes of serum IL-6,Ngb (neuroglobin of brain),S100B protein and superoxide dismutase (SOD) after cardiopulmonary resuscitation (CPR) in swine.Methods The CPR model of swine was made by a programmed electric stimulation given to myocardium to produce a ventricular fibrillation and then a CPR was given.A flock of 16 male healthy Beijing landraces pigs of 3-4 months old were divided in random number method into control group (n =6) and ulinastatin group (n =6),because there were 12 swine survived from ventricular fibrillation.The t-test was used for the statistical analysis.ELISA was used to detect the changes in levels of those biomarkers in serum at each interval as well as the pathomorphological changes in brain tissues were observed under the light microscope after HE staining.Results (1) Before ventricular fibrillation,there were no distinct differences in levels of various biomarkers in porcine serum between two groups.After ventricular fibrillation,serum IL-6,S100B protein and Ngb levels in both groups gradually increased as time elapsed,and the levels of those biomarkers in the control were significantly higher than those in the ulinastatin group (P ＜ 0.05).Serum SOD in both groups gradually reduced,and more distinct decline of biomarkers was found in the ulinastatin group (P ＜ 0.05).(2) HE staining showed the porcine brain tissues in control group had significant ischemia,degeneration and necrosis and the degree of those pathological changes in the ulinastatin group were significantly moderated.Conclusion The immediate administration of ulinastatin as CPR initiated can alleviate porcine brain tissue damage after ischemia/reperfusion (I/R).This showed a protective effect of ulinastatin against I/R injury on porcine brain.