Effect of DATS on experimental corneal neovascularization induced by corneal suture in rats cornea / 国际眼科杂志(Guoji Yanke Zazhi)
International Eye Science
;
(12): 2015-2018, 2017.
Article
in Chinese
| WPRIM
| ID: wpr-669257
ABSTRACT
AIM:
To study the effect of diallyl trisulfide (DATS) on experimental corneal neovascularization (CNV) in rats induced by corneal suture and detect the expression of vascular endothelial growth factor (VEGF) and p-AKT in rats cornea.METHODS:
The rat model of corneal neovascularization (CNV) was induced by corneal suture.Rats were randomly divided into Group Aphysiological saline control group containing DMSO (10 rats);Group B25μmol/L DATS treatment group (10 rats);Group C50μmol/L DATS treatment group (10 rats);Group D100μmol/L DATS treatment group (10 rats);Group E200μmol/L DATS treatment group (10 rats).The occurrence and development of CNV were observed by slit-lamp microscope at 7d after suture,and the area of CNV were calculated.Two weeks later,HE staining was used to observe the pathological organization form of each cornea,and RT-PCR and Western blot were used to detect the expression of VEGF mRNA and protein expression of VEGF and p-AKT between each groups.RESULTS:
The blood vessel area of Group C,D and E was compared with that of Group A,the difference was statistically significant (P<0.05);HE slice showed corneal edema,angiogenesis and inflammation infiltration situation gradually reduced comparing with the Group A,with the increase of concentration of DATS.RT-PCR showed the expression of VEGF mRNA in Group B,C,D,and E decreased compared with the Group A,and the difference was statistically significant (P<0.05).Western-blot showed that the expressions of VEGF and p-AKT in Group B,C,D and E decreased gradually compared with those in Group A,and the difference was statistically significant (P<0.05).CONCLUSION:
DATS can inhibit corneal neovascularization of the rats induced by suture.Its mechanism may be associated with suppression of VEGF secretion,down-regulation of VEGF and inactivation of p-AKT.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Prognostic study
Language:
Chinese
Journal:
International Eye Science
Year:
2017
Type:
Article
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