Correlation of transforming growth factor-β1 29C/T gene polymorphisms with adult chronic periodontitis in Uygur population of Moyu county / 新乡医学院学报

ABSTRACT

Objective To investigate the correlation between transforming growth factor-β1 (TGF-β1)29C/T(codon 10) gene polymorphisms and adult chronic periodontitis in Moyu Uygur population.Methods Three hundred samples from the region's biological sample library of chronic periodontitis were selected randomly and divided into control group periodontal healthy subjects,mild chronic periodontitis group,moderate and severe chronic periodontitis group,with 100 samples in each group.The distribution of genotype and allele of TGF-β1 29C/T (codon 10) in the subjects was detected by tetra-primer amplification refractory mutation system polymerase chain reaction (T-ARMS-PCR) method.The association between TGF-β1 29C/ T gene polymorphism and chronic periodontitis was analyzed by Chi-squared test,ordinal classification logistic regression analysis and other methods.Results There was significant differences in sex composition,age,attachment loss and probing depth among the three groups(P ＜ 0.05),and the genotype distribution was in accordance with the Hardy-Weinberg genetic balance (P ＞ 0.05).There was statistic difference in the distribution of genotype and allele frequencies among control group,mild chronic periodontitis group,moderate and severe chronic periodontitis group (x2 =12.161,8.418;P ＜ 0.05).After Bonferroni correction,there was significant difference in the distribution of genotype and allele frequencies between the control group and the moderate and severe chronic periodontitis group (x2 =11.306,8.415;P ＜ 0.016 7);there was no significant difference in the distribution of genotype and allele frequencies between the control group and mild chronic periodontitis group,mild chronic periodontitis group and moderate and severe chronic periodontitis group (P ＞ 0.016 7).Logistic regression analysis showed that the risk of chronic periodontitis in people aged 55-65 years was 85.29 times (95％ CI34.81-209.14) higher than those aged less than 35 years old,and the risk of chronic periodontitis in people with TT genotype was 2.36 times higher than those with CC genotype.Conclusion This study indicate that TGF-β1 29C/T (codon 10) gene polymorphism may contribute to the susceptibility of chronic periodontitis in Moyu county of Xinjiang Uygur population,and the age and TT genotype may be risk factors for chronic periodontitis in this population.