MAGI1 inhibits cancer cell migration and invasion of hepatocellular carcinoma via regulating PTEN / 中南大学学报(医学版)

ABSTRACT

Objective To explore the biological function and molecular mechanism of membrane associated guanylate kinase,WW and PDZ domain containing 1 (MAGI1) in hepatocellular carcinoma.Methods HepG2MAGI1 stable cell line was constructed by transfecting HepG2 cells with pcDNA3.1-MAGI1 plasmid.Wound healing and invasion assay were performed to compare the migration and invasion ability of HepG2MAGI1 and HepG2 cells.Furthermore,the expression of MAGI1 and phosphatase and tensin homolog deleted on chromosome ten (PTEN) was also examined by Western blot and the relationship was analyzed.Results The wound healing assay showed that the closure of HepG2MAGI1 cells was significantly slower than that of HepG2 cells [(90±10)% vs.(50±15)%,P＜0.05],and the invasion assay showed that the number of HepG2MAGI1 cells that passed through the matrigel was fewer than HepG2 cells (68±18 vs.150±30,P＜0.05).The protein expression level of PTEN was significantly elevated in HepG2MAGI1 cells compared with HepG2 cells (1.40±0.32 vs.0.28±0.15,P＜0.05).MAGI1 and PTEN protein expression levels were positively correlated (r=0.913,P＜0.01).Conclusion MAGI1 may inhibit the cancer cell migration and invasion of hepatocellular carcinoma via regulating PTEN.