Determination of Gabapentin and Related Substances in Gabapentin Capsules by HPLC / 中国药师
China Pharmacist
;
(12): 520-522, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-669664
ABSTRACT
Objective:
To establish an HPLC method for the determination of gabapentin and the related substances in gabapentin capsules.Methods:
A Kromasil C8 column (250 mm × 4. 6 mm,5μm)was used. The mobile phase was methanol-0. 01 mol·L-1 po-tassium dihydrogen phosphate solution (adjusting pH to 6. 9 with 2 mol·L-1 potassium hydroxide) (40∶60). The detection wave-length was 210 nm. The column temperature was 40℃ and the injection volume was 20μl. The main component and the known impuri-ty were determined by an external standard method. The unknown impurities were determined by a self-control method.Results:
The calibration curve of gabapentin was linear within the range of 2.5-20.0 mg·ml-1(r=0.999 9). The average sample recovery was 100. 74% with RSD of 0. 24%(n=5).Conclusion:
The method is simple, accurate,specific and applicable in the determination of gabapentin and the related substances in gabapentin capsules.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacist
Year:
2015
Type:
Article
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