In vitro and in vivo Antitumor Effect of Lentinan Combined with 5-FU / 中国药师
China Pharmacist
;
(12): 400-403, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-669686
ABSTRACT
Objective:
To study the in vitro and in vivo anti-tumor effect of lentinan combined with 5-FU.Methods:
MTT method was used to determine the proliferation of H22 in vitro, and the cell cycle changes were analyzed by a flow cytometry. The in vivo anti-tumor effect was evaluated in Kunming mice. The tumor inhibitory rate, thymus/spleen index, IL-2, IL-6 and TNF-α were deter-mined.Results:
The in vitro results revealed that lentinan had no obvious effect on the inhibitory rate and cell cycle of the cells treated with 5-FU. In vivo results showed that lentinan at the dosage of 25 and 50 mg·kg-1 could obviously enhance the anti-tumor effect of 5-FU(P<0. 05). Furthermore, lentinan could increase the thymus/spleen index of the tumor-bearing mice.Conclusion:
Lentinan com-bined with 5-FU has synergistic effects on anti-tumor activity through nonspecific immune stimulation rather than the direct killing of tumor cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacist
Year:
2015
Type:
Article
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