Determination of Four Flavonoids in Radix Hedysari by HPLC / 中国药师

ABSTRACT

Objective:To establish an HPLC method for the determination of calycosin-7-glucoside, genistein, formononetin and medicarpin in Radix Hedysari. Methods: The sample was refluxed with methanol in a water bath. The HPLC was performed on an SunFire C18 column(250 mm × 4. 6 mm,5 μm) with acetonitrile-0. 2% H3 PO4 as the mobile phase by gradient elution. The flow rate was 1. 0 ml·min-1 and the column temperature was at 35℃. The detection wavelength was 240 nm and the sample size was 20 μl. Results:The linear range of calycosin-7-glucoside was 0.035-1.042 μg(r=0.999 6), that of genistein was 0.027-0.821 μg(r=0. 999 7), that of formononetin was 0. 031-0. 941 μg(r=0. 999 9) and that of medicarpin was 0. 025-0. 745 μg(r=0. 999 6). The average recovery of calycosin-7-glucoside, genistein, formononetin and medicarpin was 100. 32%(RSD=1. 87%), 99. 3%(RSD=1. 76%), 100. 5%(RSD=1. 48%) and 99. 2%(RSD=1. 45%)(n=6), respectively. Conclusion:The method shows short analyt-ic time, good stability and promising operation accuracy, which provides the reference for the quality control of Radix Hedysari.