Determination of Calcium Disodium Edetate in Fosaprepitant Dimeglumine for Injection by HPLC / 中国药师
China Pharmacist
;
(12): 711-713, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-669784
ABSTRACT
Objective:
To establish an HPLC method for the determination of calcium disodium edetate in fosaprepitant dimeglu-mine for injection.Methods:
The chromatographic column was Agilent Zorbax Eclipse XDB C18(250 mm ×4.6 mm,5μm). The mo-bile phase consisted of 0. 2% tetrabutylammonium hydroxide(adjusting pH to 4. 0 with phosphoric acid)-acetonitrile(85∶15). The flow rate was 1. 0 ml·min-1 . The detection wavelength was 254 nm. The chromatographic column temperature was 35℃. The injection volume was 20μl.Results:
The calibration curve was linear within the range of 26. 06-104. 24μg·ml-1(r=0. 999 8) for calcium di-sodium edetate. The average recovery was 99. 51% (RSD=0. 25%, n=9).Conclusion:
The method is simple, rapid, accurate, specific and reproducible, and can be used in the determination of calcium disodium edetate in fosaprepitant dimeglumine for injection.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacist
Year:
2015
Type:
Article
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