Determination of Six Main Flavonoids in Herba Epimedii by Self Contrast with Correction Factor / 中国药师

ABSTRACT

Objective:To determine the content of six main flavonoids in herba epimedii by self contrast with correction factor. Methods:HPLC was carried out with reversed-phase technique on an Agilent ZORBAX Eclipse XDB-C18 (150 mm × 4. 6 mm, 5 μm) column with the mobile phase consisting of acetonitrile-water with gradient elution. The detection wavelength was 268nm and the col-umn temperature was 30℃. The correction factor was established through the increase value of chromatographic peak area for the self internal standard. The relative retention time and spectrum reference method were used to determine the position of impurities. Re-sultsThe relative retention time of epimedin A1 , epimedin A, epimedin B, epimedin C and baohuoside I was 0. 750,0. 810,0. 865, 0. 939 and 1. 651, respectively. The correction factor of them was 0. 998 6, 0. 998 7, 0. 998 8, 0. 989 4 and 0. 985 6, respectively. Conclusion:The self contrast with correction factor can be use to quantitatively determine the components with the same kind of chem-istry environment. The method is simple, efficient and accurate in the quantitative analysis of multi components in Chinese medicines.