Effects of hexamethylene bisacetamide on apoptosis and the expression of bcl-2 in human mucoepidermoid carcinoma cell line EEC-1 / 实用口腔医学杂志
Journal of Practical Stomatology
;
(6)1995.
Article
in Chinese
| WPRIM
| ID: wpr-670477
ABSTRACT
Objective:
To investigate the effects of hexamethylene bisacetamide (HMBA) on apoptosis and the expression of bcl-2 in human mucoepidermoid carcinoma cell line MEC-1.Methods:
MEC-1 cells were oxposed to 2 mmol/L HMBA or 1 mg/L 5 -Flourouracil (5 -FU ) for 72 hours. A poptosis index, the expression of bcl-2 protein and bcl-1 mRNA were determined by morphologic observation, terminal dUTP nick and labeling(TUNEL) stain, immunohistochemistry, in situ hybridization and image analysis.Results:
Apoptosis indexes(%) in HMBA treated. 5-Fu treated and control cells were 68.5, 23.6 and 2.3 respectevely. The expression of bcl-2 protein and bcl-2 mRNA in HMBA treated cells was higher than in 5-Fu treated or the control cells(p
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Practical Stomatology
Year:
1995
Type:
Article
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