Changes of [Ca~(2+)]i and protein kinase C levels in the process of adriamycin induced apoptosis of MEC-1 cells / 实用口腔医学杂志

ABSTRACT

Objective: To study the significance of intracellular free calcium levels ([Ca 2+]i) and protein kinase C(PKC) levels in the process of human salivary gland mucoepidermoid carcinoma MEC-1 cell apoptosis induced by adriamycin. Methods: MEC-1 cells were treated with adriamycin at 10 ?mol/L for 30 s～24 h.Apoptosis of the cells was investigated by light and electron microscopy, agarose gel electrophoresis and flow cytometry. [Ca 2+]i was determined by flow cytomerty, PKC by Bardford method. Results: The results showed that MEC-1 cells presented classic morphologic features of apoptosis. [Ca 2+]i in the treated cells was increased from (36.63?0.61) nmol/L to (84.00?0.45) nmol/L after 30 s～24 h treatment,while that in the control cells was 17.43?0.47 (P