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The therapeutic mechanism of adenovirus mediated CD/5-FC system in the treatment of oral squamous carcinoma cells / 实用口腔医学杂志
Journal of Practical Stomatology ; (6)2000.
Article in Chinese | WPRIM | ID: wpr-670629
ABSTRACT

Objective:

To study the therapeutic mechanism of adenovirus mediated cytosine deaminase /5-fluorocytosine (AdCMVCD/ 5-FC) suicide gene system in the treatment of oral squamous carcinoma cells in vitro.

Methods:

3H-thymidine ( 3H-TdR) incorporation assay, flow cytometry (FCM), transmission electron microscope and TUNEL (TdT-mediated dUTP Nick End Labeling)assay were used to detected the changes of Tca8113 cells after the treatment with AdCMVCD/5-FC system.

Results:

After treatment with CD/5-FC system, 3H-TdR incorporation rate of the cells decreased and significantly decreased between different MOI (multiple of infection) at 5-FC 10 -3 mol/L (P0.05); many apoptotic cells were found under transmission electron microscope and the positive rate of apoptotic cells increased from (4.40?0.87)% (before treatment) to (15.80?1.55)% (P

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Journal of Practical Stomatology Year: 2000 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Journal of Practical Stomatology Year: 2000 Type: Article