The effect of different collagen concentration on the proliferation of rat fibroblasts in three-dimensional culture / 实用口腔医学杂志
Journal of Practical Stomatology
;
(6)2000.
Article
in Chinese
| WPRIM
| ID: wpr-670694
ABSTRACT
Objective:
To study the effects of different collagen conc en tration on the proliferation of rat fibroblasts.Methods:
Fibrobl asts of 1-2 days new born SD rat from back skin were isolated and seeded into D MEM containing ?=10% fetal boven serum and collagen at the concentration (mg/ml ) of 6.4,5.3and 4.0 respectively. The status and numbers of fibroblasts were observed under light microscope and analysized using ttest.Results:
The general condition of fibroblasts in 6.4 mg/ml collagen was better than that in the other groups. The processes of the cells were apparent, simila r to the status of the cells in two-dimensional culture. Vacuoles and granules in cells were observed in 5.3 mg/ml collagen. In 4 mg/ml collagen, the processe s of fibroblasts disappeared; the nuclei were karyopyknosis or dissolved. Some c ells was likely to be apoptotic. The average cell number per 200? field of micr oscope in 6.4,5.3 and 4.0 mg/ml collagen was 25,15 and 6 respectively (P
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Practical Stomatology
Year:
2000
Type:
Article
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