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The effect of different collagen concentration on the proliferation of rat fibroblasts in three-dimensional culture / 实用口腔医学杂志
Journal of Practical Stomatology ; (6)2000.
Article in Chinese | WPRIM | ID: wpr-670694
ABSTRACT

Objective:

To study the effects of different collagen conc en tration on the proliferation of rat fibroblasts.

Methods:

Fibrobl asts of 1-2 days new born SD rat from back skin were isolated and seeded into D MEM containing ?=10% fetal boven serum and collagen at the concentration (mg/ml ) of 6.4,5.3and 4.0 respectively. The status and numbers of fibroblasts were observed under light microscope and analysized using ttest.

Results:

The general condition of fibroblasts in 6.4 mg/ml collagen was better than that in the other groups. The processes of the cells were apparent, simila r to the status of the cells in two-dimensional culture. Vacuoles and granules in cells were observed in 5.3 mg/ml collagen. In 4 mg/ml collagen, the processe s of fibroblasts disappeared; the nuclei were karyopyknosis or dissolved. Some c ells was likely to be apoptotic. The average cell number per 200? field of micr oscope in 6.4,5.3 and 4.0 mg/ml collagen was 25,15 and 6 respectively (P

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Journal of Practical Stomatology Year: 2000 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Journal of Practical Stomatology Year: 2000 Type: Article