The effect of TIP30 on cell cycle distribution of ACC-Mcells / 实用口腔医学杂志
Journal of Practical Stomatology
;
(6)2000.
Article
in Chinese
| WPRIM
| ID: wpr-670696
ABSTRACT
Objective:
To study the effect of TIP30 gene on the cell cy cle distribution in adenoid cystic carcinoma ACC-M cells.Methods:
Wild type TIP30 gene was transfected into ACC-M cells by using lipofectamin e, the postive cell clone was selected with G418,the expression of TIP30 protei n in the cells was detected by Western blot. Cell cycle distribution was detecte d by flow cytometry.Results:
14 days after seletion anti-G418 c ell clone was obtained. Wstern blot revealed that the TIP30 protein was expresse d in the transfected cells.Flow cytometric analysis indicated that TIP30 induced a arrest of the cells in G 0-G 1 phase and led to a decrease of cell percent age in G 2-M and S phase.Conclusion:
ACC-M can stably express exogene TIP30.TIP30 can inhibit proliferation of ACC-M cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Practical Stomatology
Year:
2000
Type:
Article
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