Appraisement of multidrug resistance of a human salivary adenoid cystic carcinoma cell line / 实用口腔医学杂志
Journal of Practical Stomatology
;
(6)1995.
Article
in Chinese
| WPRIM
| ID: wpr-670756
ABSTRACT
Objective:
To establish a multidrug resistant human salivary adenoid cystic carcinoma cell line.Methods:
Salivary adenoid cystic cells of the cell line SACC were exposed to 1 ?g/ml of DDP for 48 h at the interval of one month. 6 months later, the cell line was established and named SACC/DDP. MTT method was used to study the drug resistance of SACC and SACC/DDP cells against 4 chemotherapeutic agents(MTX, PYM, VCR, MMC). Flow cytometry was adopted to study the cell cycle distribution of the cells after treatment with 4 agents at different concentrations for 72 h.Results:
The drug resistance of SACC/DDP cells against VCR,MTX,PYM and MMC was 2.9,2.4,1.1 and 1.8 times of that of SACC cells. The ratio of G0 cell number to G1 cell number in SACC/DDP cell line was smaller than that in SACC cell line after treatment with the 4 agents at different concentrations respectively.Conclusion:
The multidrug resistant human salivary adenoid cystic carcinoma cell line can be estabished by exposing parent cell line SACC periodically to DDP.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Practical Stomatology
Year:
1995
Type:
Article
Similar
MEDLINE
...
LILACS
LIS