Effect of radiation on multidrug resistance of tongue squamous cell carcinoma cell line Tca 8113 / 实用口腔医学杂志

ABSTRACT

Objective:To study radiation sensibility of tongue squamous cell carcinoma cell line Tca 8113 and its multidrug resistance(MDR)cell line Tca 8113/CBDEA.MethersMTT assay was used to determine the sensitivity of the cells to radiation.Cells were treated with 2 Gy of ?-radiation for 4 or 24 h.Real-time quantitative reverse transcriptase-polymerase chain reaction(RT-PCR)was used to detecte the expressions of mdr related genes(mdr1,mrp1,gst-?)and HTS 7000 Plus Bio Assay Reader was used to measure intracellular ADM concentration.Results:Tca 8113/CBDEA cells were 1.24-fold more radiation resistant than that of Tca 8113 cells(P0.05),while increased 24 h after radiation(P