Role of apoptosis related gene Bcl-2 and Bax during periodontal ligament remodeling in rats / 实用口腔医学杂志

ABSTRACT

Objective: To study the influence of occlusal force changes on apoptosis related gene Bcl-2 and Bax in rat periodontal ligament fibroblasts (PDLF). Furthermore, to explore the possible mechanism in remodeling of periodontal ligaments. Methods: Animal model of occlusal force changes was established by extracting the right first, second and third maxillary molars in male SD rats. Rats were sacrificed at 6,12 hours and 1, 2, 3, 5, 7, 14 and 28 days after teeth extraction(n=6), and their alveolar bone tissues were harvested. HE staining was used to observe the morphologic changes of alveolar bone tissues and immunohistochemistry were used to detect Bcl-2 and Bax expressions in PDLF. The rats of normal occlusal forces were used as control(n=6). The results were assigned a mean score based on the expressions and the intensity of cell positively stained for Bcl-2 and Bax. Results: Animal model of occlusal force changes was successfully established. HE staining showed that the control group had more pycnotic periodontal ligament, orderly aligned fibres, and flatter alveolar bone than model group. Immunohistochemical staining showed that expression of Bax reached its peak at 12 h after extracting the teeth(267.00?7.82), significantly higher than that of control group(25.67?4.97,P