Effects of the cyclic uniaxial compressive stress on RKIP and its mRNA in the rat condylar chondrocytes / 实用口腔医学杂志

ABSTRACT

Objective: To investigate the effects of the cyclic uniaxial compressive stress on RKIP and its mRNA in the rat condylar chondrocytes. Methods:The third-passage chondrocytes were harvested from the mandibular condyles of 2-day-old rats, and a cellular compressive stress device (self-made four-point bending system. Patent No 01129166.4 & 01256849.x) was used to apply stress on cells at 4 000 ?strain ( 0.5 Hz frequency) for 0, 15, 30, 60, 120 and 240 min. The effects of the cyclic uniaxial compressive stress on RKIP and its mRNA in the rat mandibular condylar chondrocytes were examined by REAL-TIME PCR and Western blot. Results:The results showed that the expression of RKIP and its mRNA changed obviously with 4 000 ?strain compressive stress loading, there are reverse tendency in the expression between RKIP and its mRNA. RKIP mRNA significantly increased at 30min (P