Effect of short exposure to high levels of fluoride on cellular structural and the expression of BMP-4 in ameloblasts of mouse molar / 实用口腔医学杂志

ABSTRACT

Objective:To study the effect of short exposure to high levels of fluoride on structure and the BMP-4 expression of ameloblasts of mouse molar and explore the possible formation mechanisms of dental fluorosis. Methods:32 4-day-old ICR mice were randomly divided into two groups. The experimental animals and the control animals were shared equal numbers in each group. The experimental animals were received a single intraperitoneal dose of 20 (n=8) and 10 (n=8) mg NaF/kg(body weight) respectively,equal doses of NaCl were given to the controls (n=8 for each group). The injected volume was kept constant (10 ?l/g). After 24 h all mice were sacrificed. HE and immunohistochemical stainings were used to observe the structural changes and the expression of BMP-4 in ameloblasts of mouse molar at different stages. SPSS 13.0 software package was used to analyze the data. Results:The early and late secretory/transitional ameloblasts were sensitive to a short-lasting but high dose of F-. The immunohistochemistry results demonstrated that in experimental animals,the expression of BMP-4 was weaker than that in control animals with the significant differences(P