Preparation and Drug Release in vitro of Danshensu Liposomes / 中国药师
China Pharmacist
;
(12): 1649-1651,1660, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-671167
ABSTRACT
Objective:
o prepare Danshensu liposomes and investigate drug release characteristics in vitro.Methods:
Danshensu liposomes were prepared by a reverse-phase evaporation method. The encapsulation efficiency was used as the index, an orthogonal test was adopted to investigate the effect of concentration of soybean lecithin, ratio of lipid-Danshensu and pH value of solution on the preparation procedure of Danshensu liposomes. The particle size of the liposomes was also investigated by a transmission electron micro-scope ( TEM) . The concentration of Danshensu was determined by HPLC, and the difference of release characteristics in Danshensu li-posomes and Danshensu solution was measured by a dialysis method.Results:
The optimum preparation technology was as followsthe concentration of soybean lecithin was 40 mg·ml-1 ,the ratio of drug-lipid was 1 10,and the pH value of solution was 6. 6. The mor-phology of the prepared liposomes showed spheric structure with uniform diameter, and the average particle size was ( 174 ± 36 ) nm and the encapsulation efficiency was 38. 9%. The linear range of Danshensu was 2. 0-20. 0 mg·L-1(r=0. 9984). The drug release of liposomes in vitro was slower than that of free Danshensu solution in 24 h.Conclusion:
Danshensu liposomes with fine morphology have sustained release property.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacist
Year:
2015
Type:
Article
Similar
MEDLINE
...
LILACS
LIS