Etiological analysis of diarrhea patients visiting enteric disease clinics in Beijing / 中华传染病杂志

ABSTRACT

Objective To investigate the etiological characteristics of diarrhea patients visiting enteric disease clinics in Beijing .Methods A total of 595 stool samples were collected among outpatients with diarrhea vistiting enteric disease clinics at two sentinel hospitals from July 2013 to June 2014 . Diarrheagenic Escherichia coli (E . coli) , Vibrio parahemolyticus , O1 or O139 Vibrio cholerae , Salmonella and Shigella were isolated according to standard methods . And rotavirus , norovirus , astrovirus and enteric adenovirus were identified by molecular techniques .The characteristics of population and temporal distribution , and serotypes of these pathogens were analyzed using descriptive epidemiological method .Chi‐square test was used for comparison between groups .Results Totally 128 bacterial strains were isolated from 595 samples ,and the detection rate was 21 .5% .Diarrheagenic E .coli was most common pathogenic bacteria (11 .4% ,68/595) ,followed by Vibrio parahemolyticus (6 .9% , 41/595) ,Salmonella (2 .4% ,14/595) and Shigella (2 .2% ,13/595) .No V ibrio cholerae was detected . One hundred and twelve viral strains were detected from all samples ,and the positive rate was 18 .8% . Norovirus was most common viral pathogen (9 .1% ,54/595) ,followed by rotavirus (8 .7% ,52/595) , astrovirus (1 .8% , 11/595 ) and enteric adenovirus (0 .7% , 4/595) . Enteropathogenic E .coli , enterotoxigenic E .coli and enteroadhesive E .coli were the most common types of diarrheagenic E .coli . The most common serotype of Vibrio parahaemolyticus was O4∶K8 .The detection rate of bacterial pathogens reached the peak from June to September ,while the highest detection rate of viral pathogens was found from November to the next March .Conclusion Norovirus and rotavirus are also the main pathogens of the diarnhea patients visiting enteric clinics ,which should be paid enough attention .