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Analysis of human B cell response to recombinantLeishmaniaLPG3
Asian Pacific Journal of Tropical Biomedicine ; (12): 615-619, 2015.
Article in Chinese | WPRIM | ID: wpr-672428
ABSTRACT

Objective:

To evaluate the capability of recombinant Leishmania LPG3 and its fragments in the activation of B cells.

Methods:

In the present study, human B cells were purified from peripheral blood of 10 adult healthy subjects using magnetic-activated cell sorting technique. Subsequently, purified B cells were treated with recombinant LPG3, and itsN-terminal and C-terminal fragments at different concentrations (2, 10 and 20 μg/mL). B cell activation was assessed through expression of CD69 molecule by flowcytometry and secretion of IL-6, TNF-αα and IL-10 cytokines via enzyme-linked immunosorbent assay following treatment with recombinant antigens.

Results:

Our results showed that while the recombinant LPG-3 could significantly increase the production of IL-6 and TNF-α (P<0.05) in B cells, it had no effect on the secretion of IL-10 by B cells.

Conclusions:

Our study indicated that recombinant LPG-3 and especially itsN-terminal fragment could stimulate B cell response as an important immune response component against leishmaniasis. Thus, it seems that it can be considered as an effective adjuvant in vaccine developments against leishmaniasis.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Asian Pacific Journal of Tropical Biomedicine Year: 2015 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Asian Pacific Journal of Tropical Biomedicine Year: 2015 Type: Article