HPLC-TOF/MS profile and nitric oxide scavenging activity of Orthosiphon stamineus leaf extracts
Asian Pacific Journal of Tropical Biomedicine
;
(12): 1436-1439, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-672497
ABSTRACT
Objective:
The aim of the present study is to develop liquid chromatography (LC)/Time-of-flight mass spectrometry (TOF/MS) profile for methanol and water extracts of Orthosiphon stamineus leaf using SEN and RA as flavonoid and non-flavonoid polyphenolic markers in the extracts. The study also evaluates in vitro nitric oxide radical scavenging effect of the extracts.Method:
Orthogonal Time of Flight Mass Spectrometer equipped with HPLC separation module was used in the analyses of the extract. The in vitro nitric oxide scavenging activity of the extracts was measured according to the method described by Rao.Results:
The qualitative analysis of the extracts performed with HPLC-TOF/MS confirmed the presence sinensitin (SEN) and rosmarinic acid (RA) in the extracts. The extracts showed in vitro nitric oxide scavenging activities.Conclusions:
The HPLC-TOF/MS method could be employed for quality determination of herbal medicinal products and formulations containing O. stamineus. The extracts may play a significant role in prevention of degenerate disease due to its ability to scavenge nitric oxide radical.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Qualitative research
Language:
Chinese
Journal:
Asian Pacific Journal of Tropical Biomedicine
Year:
2012
Type:
Article
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