Influence of volatile constituents of fruit peels of Citrus reticulata Blanco on clinically isolated pathogenic microorganisms under In-vitro

ABSTRACT

Objective: To investigate the antimicrobial activity of volatile constituents of fruit peels of Citrus reticulata Blanco on clinically isolated pathogenic microorganisms. Methods: Extraction of volatile oil was carried out by Clevenger’s apparatus. Volatile chemical components were measured by GC-MS. Antimicrobial activity was carried by Agar well diffusion assay with reference to standard fluconazole and tetracycline. Results: The chemical composition of volatile oil of the fruit peels of Citrus reticulata Blanco (Rutaceae) of Delhi Region was composed mainly monoterpenes (99.1 %) constituting l-limonene (92.4 %), γ-terpene (2.6 %) andβ-phellandrene (1.8 %). The volatile oil showed antibacterial and antifungal activities against the clinically isolated pathogenic microbial strains Escherichia coli, Staphylococcus aureus, Aspergillus flavus, Aspergillus niger, Aspergillus fumigatus and Candida albicans under in vitro condition. Conclusions: The potential antimicrobial activity of volatile oil present in fruit peels of C. reticulata can be useful for treatment of skin disorder and/or in aroma. Therapy, it can be incorporated into cosmetic formulations.