Preliminary Phytochemical, UV-VIS, HPLC and Anti-bacterial Studies on Gracilaria corticata J. Ag

ABSTRACT

Objective: The present study aimed to investigate the preliminary phytochemical analysis and UV-VIS, HPTC profiling and the antibacterial activity of Gracilaria corticata J. Ag extracts against the Gram positive and Gram negative bacteria. Methods: Preliminary phytochemical screening was carried out by Harborne method. The G. corticata extracts were tested against bacteria by the agar disc diffusion method. Results: The results of the presence study showed the presence of alkaloids, steroids, phenolic groups, saponins, tannin, flavonoids, terpenoids, glycosides and sugars. Proteins, xantoproteins, coumarins and catechin did not show any positive result for their presence in any of the six extracts of Gracilaria corticata tested. The result of the present study revealed the various behavior character of Gracilaria corticata crude drug. The UV-VIS spectrum profile of Gracilaria corticata methanolic, petroleum ether, benzene and aqueous extracts profiles were recorded. The HPLC profile of Gracilaria corticata petroleum ether benzene and aqueous extracts were tabulated. The maximum (9/12 bacterial pathogens) degree of antibacterial activity was observed in isopropanol soxhlet extracts followed by isopropanol cold extracts (7/12 bacterial pathogens). Conclusion: The results of the present study showed that G. corticata may be rich sources of phytoconstituents which can be isolated and further screened for different kinds of biological activities, depending on their reported therapeutic uses.