Cytoprotective and anti-inflammatory effects of kernel extract from Adenanthera pavonina on lipopolysaccharide-stimulated rat peritoneal macrophages

ABSTRACT

Objective:To investigate mechanism of anti-inflammatory activity ofAdenanthera pavonina (A. pavonina) extracts.Methods:Rat peritoneal macrophages were treated with different concentrations of lipopolysaccharide andH2O2 in the presence and absence of kernel extract from A. pavonina.Nitric oxide, superoxide anion generation, cell viability and nuclear fragmentation were investigated.Results:The pre-treatment of kernel extract fromA. pavonina suppressed nitric oxide, superoxide anion, cell death, nuclear fragmentation in lipopolysaccharide andH2O2 stimulated or induced macrophages, respectively.Conclusions:These results suggest thatA. pavonina extract suppresses the intra cellular peroxide production.